paraffin human breast tumor tissue array  (BioChain Institute)

Journal: Journal of cancer science & therapy

Article Title: Knockdown of the Inhibitor of Apoptosis BRUCE Sensitizes Resistant Breast Cancer Cells to Chemotherapeutic Agents

doi: 10.4172/1948-5956.1000335

Figure Lengend Snippet: IHC of BRUCE on normal breast tissue ( A ) and primary breast tumors ( B–D ) on paraffin array slides with tumor tissue outlined in red circles and adjacent normal tissues in blue circles; scale bar: 20 mircometer. Statistical analysis of BRUCE IHC staining ( E ) was based on clinic scoring criteria described in the Methods. The values represent average scores of normal vs tumor tissues. Variation represents standard error of the mean (SEM). Student’s t -test, P =0.013; ( p <0.05 was regarded significant; one-tailed test).

Article Snippet: Ethics Statement The paraffin human breast tumor tissue array was purchased from BioChain ( http://www.biochain.com ).

Techniques: Immunohistochemistry, One-tailed Test

Journal: Cell Death & Disease

Article Title: CtBP maintains cancer cell growth and metabolic homeostasis via regulating SIRT4

doi: 10.1038/cddis.2014.587

Figure Lengend Snippet: CtBP regulates glutaminolysis via repressing SIRT4. ( a and b ) SIRT4 expression was determined in MCF-7 cells with CtBP knockdown (CtBP KD) or CtBP overexpression (CtBP OE) by RT-PCR and western blotting. CtBP was overexpressed as fusion protein with GFP tag. ( c ) Left, representative immunohistochemistry (IHC) staining for CtBP and SIRT4 in human breast normal tissues and breast tumor tissues (scale bar: 25 um); middle and right, histograms to show CtBP and SIRT4 staining in both normal tissues and breast tumor tissues. The columns represent the average staining intensity by each antibody in IHC assays. ( d ) ChIP assay of CtBP binding at SIRT4 promoter. A neighbor region of SIRT4 gene without transcripts (Non-pro) was used as negative binding control region, and nonspecific IGG (NSIgG) was used as negative control for chromatin pull down. The binding was shown as percentage to input. ( e ) Glutamine consumption was determined in MCF-7 cells with different treatments as indicated. The measurements were performed after 72 h for gene knockdown and the BPTES dosage was 10 uM. ( f ) GDH activity was measured in cells treated by the conditions as indicated. The measurements were performed 72 h after treatment. ( g ) Ammonia production in response to the conditions as indicated in MCF-7 cells. ( h and i ) The measurement of both the medium pH and intracellular acidity of MCF-7 cells with conditions as indicated. The error bars represent the S.D. of three independent replicates. * P <0.05, ** P <0.01. In ( e , f and g ) the P- values were calculated between the control sample and the indicated sample, respectively

Article Snippet: Formalin-fixed, paraffin-embedded human normal breast and tumor breast tissue arrays were purchased from Biomax (Rockville, MD, USA) with patients's information available.

Techniques: Expressing, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Staining, Binding Assay, Negative Control, Activity Assay

Journal: International journal of cancer

Article Title: Neuropilin-1 is differentially expressed in myoepithelial cells and vascular smooth muscle cells in preneoplastic and neoplastic human breast: a possible marker for the progression of breast cancer.

doi: 10.1002/ijc.10611

Figure Lengend Snippet: FIGURE 5 – Detection of mRNA for NRP-1 in formalin-fixed paraffin-embedded human breast tissue sections by in situ hybridization using DIG labeled PCR generated exon or intron specific probes. The purple color indicates the expression of NRP-1 mRNA. Sections were counter stained with nuclear first red. (a) Normal breast tissue section illustrating negative staining, which was observed in most normal breast tissue sections. Magnification 300. (b) Benign duct adjacent to the invasive carcinoma cells. Arrows, myoepithe- lial cells expressing NRP-1 mRNA; arrow heads, epithelial cells that do not express NRP-1. Magni- fication 300. (c) Negative control. Section was hybridized with an intron-specific DIG-labeled PCR generated probe. Magnification 400. (d) Blood vessels from invasive carcinoma section. The arrows point to vascular SMC expressing NRP-1 mRNA. Arrow heads, endothelial cells. Magnification 300.

Article Snippet: Formalin-fixed and paraffin-embedded human breast tissue array slides (Imgenex Co., CA.) were also analyzed for the present studies.

Techniques: In Situ Hybridization, Labeling, Generated, Expressing, Staining, Negative Staining, Negative Control